Skip to main content
Back to Glossary
Vectors, ES Cells & Delivery

Selection Markers (NeoR, PuroR)

Genes incorporated into targeting vectors to identify and isolate cells that have successfully integrated a genetic modification. Common markers include neomycin resistance (NeoR) and puromycin resistance (PuroR).

Overview

Selection markers are genes incorporated into targeting vectors to identify and isolate cells that have successfully integrated a genetic modification. Common markers include neomycin resistance (NeoR) and puromycin resistance (PuroR), which confer survival advantages under antibiotic selection.

Frequently Asked Questions

Can selection markers be removed after targeting?

Yes. Markers are often flanked by LoxP or FRT sites for removal via Cre or Flp recombinase once correctly targeted clones are established.

Related Services

Related Terms

Targeting Vector (Homology Arms)

A synthetic DNA construct designed to introduce specific genetic modifications into a genome through homologous recombination or HDR. It contains sequences known as homology arms that align precisely with the genomic target locus.

Flp-FRT System

A yeast-derived site-specific recombination mechanism enabling targeted DNA excision, inversion, or integration through the interaction of Flp recombinase with FRT sites. It serves as an orthogonal complement to Cre-lox in mammalian genetics.

More in Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)Blastocyst Injection / Chimera ProductionPronuclear Injection (Transgenics)Single-Copy IntegrationLarge-Fragment / BAC Targeting
Sanger / NGS Validation of JunctionsView All TermsSingle vs Double Humanized Targets

Need Help with Your Mouse Model Project?

Our scientific consultants can help you understand the best approach for your research goals.

Request a QuoteContact Us