We build the exact model your study needs.

Designed and delivered by ingenious targeting laboratory. Quote in 24 hours.

Get a Quote
Skip to main content
Uncertain NIH funding? Start now, pay later.
Back to Glossary
Vectors & Delivery

Blastocyst Injection / Chimera Production

The process of introducing genetically modified cells into a developing mouse blastocyst to create a chimera, an organism composed of cells from both the host embryo and the engineered cells.

Overview

Blastocyst injection is the process of introducing genetically modified cells into a developing mouse blastocyst to create a chimera, an organism composed of cells derived from both the host embryo and the engineered cells. This step is essential for transferring targeted genetic modifications into the germline.

Frequently Asked Questions

How do you identify high-contribution chimeras?

Chimeric offspring typically display coat color patterns reflecting targeted clone contribution vs. host embryo strain, which can be visually assessed at birth.

Related Services

Related Terms

Germline Transmission

The process by which a genetic modification introduced into an organism is passed on to its offspring through reproductive (germ) cells. It confirms that the engineered change is stably integrated into the genome and can be inherited by future generations.

Germline Confirmation / Coat Color Markers

Ensures that a targeted genetic modification has been successfully passed through reproductive cells to offspring. Coat-color markers serve as a visible indicator of targeted clone contribution in chimeric founder animals.

More in Vectors & Delivery

Targeting Vector (Homology Arms)Selection Markers (NeoR, PuroR)Pronuclear Injection (Transgenics)Single-Copy IntegrationLarge-Fragment / BAC Targeting
Baseline PhenotypingView All TermsBreeding Scheme (Hetero × Hetero, etc.)