Vectors, ES Cells & Delivery

Blastocyst Injection / Chimera Production

The process of introducing genetically modified cells into a developing mouse blastocyst to create a chimera—an organism composed of cells from both the host embryo and the engineered cells.

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Overview

Blastocyst injection is the process of introducing genetically modified cells into a developing mouse blastocyst to create a chimera—an organism composed of cells derived from both the host embryo and the engineered cells. This step is essential for transferring targeted genetic modifications into the germline.

Frequently Asked Questions

How do you identify high-contribution chimeras?

Chimeric offspring typically display coat color patterns reflecting ES cell contribution vs. host embryo strain, which can be visually assessed at birth.

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Related Terms

Germline Transmission

The process by which a genetic modification introduced into an organism is passed on to its offspring through reproductive (germ) cells. It confirms that the engineered change is stably integrated into the genome and can be inherited by future generations.

Germline Confirmation / Coat Color Markers

Ensures that a targeted genetic modification has been successfully passed through reproductive cells to offspring. Coat-color markers serve as a visible indicator of ES cell contribution in chimeric founder animals.

More in Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)Selection Markers (NeoR, PuroR)Pronuclear Injection (Transgenics)Single-Copy Integration Large-Fragment / BAC Targeting

Baseline Phenotyping View All Terms Breeding Scheme (Hetero × Hetero, etc.)

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