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Overview
Large-fragment or BAC (bacterial artificial chromosome) targeting involves introducing extended genomic DNA segments—often spanning 100–300 kilobases—into the mouse genome to preserve native gene context and regulatory elements.
Frequently Asked Questions
When should BAC targeting be used?
BAC targeting is particularly valuable for humanized gene models, reporter lines, rescue constructs, and multigene loci where cDNA constructs cannot capture regulatory complexity.
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