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Vectors, ES Cells & Delivery

Targeting Vector (Homology Arms)

A synthetic DNA construct designed to introduce specific genetic modifications into a genome through homologous recombination or HDR. It contains sequences known as homology arms that align precisely with the genomic target locus.

Overview

A targeting vector is a synthetic DNA construct designed to introduce specific genetic modifications into a genome through homologous recombination or HDR. It contains sequences known as homology arms that align precisely with the genomic target locus, enabling accurate exchange or insertion of DNA.

Frequently Asked Questions

How long should homology arms be?

Homology arms typically range from 1–10 kilobases for ES cell targeting. Longer arms generally increase recombination efficiency.

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Related Terms

Homologous Recombination

A precise DNA repair and genetic engineering mechanism that uses a homologous sequence as a template to exchange or replace genetic material. It allows for accurate correction or insertion of DNA sequences at defined genomic loci.

Homology-Directed Repair (HDR)

A high-fidelity DNA repair mechanism that uses a homologous DNA sequence as a template to accurately fix double-strand breaks. Unlike NHEJ, HDR ensures precise integration or correction of genetic material.

Selection Markers (NeoR, PuroR)

Genes incorporated into targeting vectors to identify and isolate cells that have successfully integrated a genetic modification. Common markers include neomycin resistance (NeoR) and puromycin resistance (PuroR).

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