Akt1 Knockin mouse models
As the dominant AGC kinase isoform in most tissues, Akt1 is the mouse ortholog of human AKT1 and encodes a PH domain kinase recruited to PIP3 generated by class I PI3K, activated by PDK1 phosphorylation of T308 in the activation loop and mTORC2 phosphorylation of S473 in the hydrophobic motif. Active AKT1 phosphorylates TSC2, PRAS40, GSK3 beta, BAD, and FOXO factors, coupling growth factor input to mTORC1 output, glycogen metabolism and apoptotic threshold. The recurrent E17K substitution in the PH domain locks the kinase at the membrane and drives Proteus syndrome and multiple carcinomas, so a conditional knockin of that codon addresses hyperactivation while a floxed null addresses requirement. Homozygous null mice are viable but growth restricted, and Akt2 and Akt3 compensate in muscle, liver and brain respectively, so isoform-specific conclusions demand compound alleles. Pair allele class to readout: null for dependency, E17K knockin for inhibitor pharmacodynamics.
Akt1 knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Akt1 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Akt1 knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Akt1-C224S | Knockin | point mutantion mice | embryo cryopreservation | KI 215096 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Akt1 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Akt1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Akt1 Knockin mouse models are available?
When Akt1 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Akt1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Akt1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Akt1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Akt1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.