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Ang Knockout mouse models
Angiogenin, the product of mouse Ang and ortholog of human ANG (also called RNASE5), is a secreted RNase A superfamily ribonuclease whose His13, Lys40, His114 catalytic triad supports weak but obligatory cleavage of tRNA anticodon loops, generating stress-induced tiRNA fragments that displace eIF4G and reprogram translation initiation. Under basal conditions the enzyme is clamped by cytosolic RNH1, and during stress it is internalized by motor neurons through syndecan and neuropilin-1 before translocating to the nucleolus to sustain 45S rRNA transcription. Amyotrophic lateral sclerosis and frontotemporal dementia alleles such as K40I and C39W are catalytically hypomorphic, so a conditional null addresses cell-autonomous requirement while a patient point knockin tests whether diminished ribonucleolytic output alone drives motor neuron vulnerability, and humanization enables recombinant protein dosing work. Because the mouse locus is a tandem cluster spanning Ang2 through Ang6, single-gene deletion is buffered; pair Chat-Cre or Gfap-Cre conditionals with neuromuscular junction and survival endpoints.
A conventional Ang knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ang Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ang knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ang-KO | KO | KO | SPERM | KO 201392 | Inquire |
| Exon 2 of Ang gene was deleted to generate Ang knockout mice. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ang Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ang allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ang Knockout mouse models are available?
When Ang Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ang knockout embryonic lethal in mice?
It depends on background and allele design. Some Ang germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ang animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ang?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Citations
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Breed this line with ingenious targeting laboratory
Send the Ang Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.