Apc Conditional Knockout mouse — intestinal specific context
Apc, the mouse ortholog of human APC, encodes the large scaffold that nucleates the beta-catenin destruction complex, using 15 amino acid and 20 amino acid repeats to bind beta-catenin and SAMP repeats to bind AXIN1 and AXIN2, thereby positioning CK1 alpha and GSK3 beta for the priming and processive phosphorylations that create the beta-TrCP degron. Independently, the armadillo repeat region and C-terminal EB1 and DLG binding sites control microtubule plus end dynamics and mitotic spindle fidelity, so truncating alleles produce both Wnt hyperactivation and chromosomal instability. Mutation position matters enormously: the mutation cluster region retains some repeats and yields intermediate signaling, which is why allelic series such as Min, 1638N and 1322T give different tumor multiplicities. Homozygous null is embryonic lethal, mandating floxed exon 14 or 15 designs with tissue-restricted Cre for intestinal, hepatic or mammary work. Match truncation position to the desired Wnt setpoint rather than assuming all Apc alleles are equivalent.
A floxed Apc allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For intestinal work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on intestine while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Apc-Flox | Conditional Knockout | KO/CKO mice, disease model mice | live | CKO 200013 | Inquire |
Designing a Apc Conditional Knockout allele
Conditional deletion is the only practical route for Apc in adult tissue, but note what the standard floxed exon 14 design actually produces: Cre excision creates a frameshift and a truncated product retaining the amino-terminal oligomerization region and some 15 amino acid repeats, not a clean null, so the allele models human truncating mutations rather than protein absence. Driver choice sets the phenotype more than the allele does. Lgr5-EGFP-IRES-CreERT2 restricts loss to crypt base columnar cells and yields discrete adenomas, whereas Villin-CreERT2 or AhCre deletes across the whole epithelium and kills animals within days from crypt hyperproliferation and malabsorption, so titrate tamoxifen for mosaicism. Read out nuclear beta-catenin, Axin2 and Lgr5 in situ, crypt fission counts, and Wnt-ligand-independent growth of derived organoids in medium lacking R-spondin.
Pricing and quotes
The Apc Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Apc allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Apc Conditional Knockout mouse models are available?
When Apc Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Apc knockout embryonic lethal in mice?
It depends on background and allele design. Some Apc germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for intestine specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to intestine specific, so we start driver selection there.
Do you ship live Apc animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Apc?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.