Apc Knockout mouse models
Apc, the mouse ortholog of human APC, encodes the large scaffold that nucleates the beta-catenin destruction complex, using 15 amino acid and 20 amino acid repeats to bind beta-catenin and SAMP repeats to bind AXIN1 and AXIN2, thereby positioning CK1 alpha and GSK3 beta for the priming and processive phosphorylations that create the beta-TrCP degron. Independently, the armadillo repeat region and C-terminal EB1 and DLG binding sites control microtubule plus end dynamics and mitotic spindle fidelity, so truncating alleles produce both Wnt hyperactivation and chromosomal instability. Mutation position matters enormously: the mutation cluster region retains some repeats and yields intermediate signaling, which is why allelic series such as Min, 1638N and 1322T give different tumor multiplicities. Homozygous null is embryonic lethal, mandating floxed exon 14 or 15 designs with tissue-restricted Cre for intestinal, hepatic or mammary work. Match truncation position to the desired Wnt setpoint rather than assuming all Apc alleles are equivalent.
Apc null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Apc Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Apc knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Designing a Apc Knockout allele
A constitutive null is only informative in heterozygosity here. Homozygous Apc-null embryos arrest before gastrulation completes, so the homozygous state is accessible only in embryoid bodies, blastocyst outgrowths, or derived ES lines. The heterozygote is the useful animal: somatic loss of the remaining allele drives multifocal adenomas, giving a quantitative tumor multiplicity readout that is unusually sensitive to genetic background. Modifier effects at Pla2g2a mean C57BL/6 and AKR or BALB/c congenics differ several fold in polyp count, so fix and report the background before comparing cohorts. Expect disease concentrated in small intestine rather than colon, which limits fidelity to human familial adenomatous polyposis and argues for adding a colitis or azoxymethane challenge. Score polyp number and diameter by region under a dissecting scope, confirm loss of heterozygosity by sequencing microdissected lesions, and stain for nuclear beta-catenin.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Apc Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Apc allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Apc Knockout mouse models are available?
When Apc Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Apc knockout embryonic lethal in mice?
It depends on background and allele design. Some Apc germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Apc animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Apc?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.