Arnt Conditional Knockout mouse models
Arnt, the mouse ortholog of human ARNT, encodes HIF1 beta, the obligate class II bHLH-PAS dimerization partner that licenses DNA binding by HIF1 alpha, EPAS1 and the aryl hydrocarbon receptor, contacting hypoxia response elements or xenobiotic response elements only as a heterodimer and recruiting p300 and CBP through partner transactivation domains. Because ARNT is constitutively expressed and not oxygen regulated, it functions as the shared limiting hub where hypoxic and xenobiotic transcriptional programs converge and compete. Germline deletion is embryonic lethal near midgestation from failed placental vascularization and yolk sac angiogenesis, so conditional alleles are essential for adult work in hepatocytes, pancreatic beta cells, keratinocytes and hematopoietic compartments. Because Arnt2 substitutes in neurons and some endocrine tissues, CNS phenotypes require compound conditional genetics to avoid false negatives. Deleting Arnt collapses the entire hypoxic and AHR axis simultaneously, so when isoform-specific questions matter, target Hif1a or Epas1 directly instead of this shared partner.
Tissue restricted knockout of Arnt keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Arnt Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Arnt conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Arnt-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 240594 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Arnt Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Arnt allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Arnt Conditional Knockout mouse models are available?
When Arnt Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Arnt knockout embryonic lethal in mice?
It depends on background and allele design. Some Arnt germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Arnt animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Arnt?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.