Axin2 Knockin mouse models
Axin2, mouse ortholog of human AXIN2 and also called conductin, encodes a destruction complex scaffold structurally parallel to AXIN1, with RGS and DIX domains supporting APC and Dishevelled interactions, but distinguished by being a direct Wnt target gene, which makes it both a negative feedback brake and the field standard transcriptional reporter of pathway activity. Reporter knockin alleles such as Axin2-LacZ or Axin2-CreERT2 exploit that property to mark and lineage trace Wnt responsive stem cells in intestine, bone, liver and hair follicle, so allele choice here is frequently about readout rather than loss of function. Human truncating variants cause oligodontia with colorectal cancer predisposition, and null mice are viable with craniosynostosis and altered bone mass, allowing constitutive alleles. Because Axin1 is the more abundant scaffold and buffers loss, expect modest signaling change from Axin2 deletion alone. Match design to purpose: reporter or CreERT2 knockins for lineage tracing, conditional nulls layered onto Axin1 alleles for feedback dissection.
Knockin designs at Axin2 support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Axin2 Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Axin2 knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Axin2 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Axin2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Axin2 Knockin mouse models are available?
When Axin2 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Axin2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Axin2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Axin2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Axin2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.