Bak1 Knockout mouse models
Bak1 encodes BAK, the mouse ortholog of human BAK1 and one of two obligate multidomain effectors of mitochondrial outer membrane permeabilization. Unlike BAX, BAK resides constitutively in the outer membrane anchored by its alpha9 transmembrane helix, and activation begins when a BH3-only ligand such as BID, BIM, or PUMA transiently engages the canonical hydrophobic groove, triggering separation of the core and latch domains, exposure of the alpha6 dimerization interface, and assembly of BH3-in-groove homodimers that oligomerize into lipidic pores releasing cytochrome c and SMAC. Because Bax provides near-complete functional overlap, single Bak1 nulls are viable and largely unremarkable, and only Bax Bak1 double deficiency reveals the full phenotype, including persistent interdigital webbing, imperforate vaginal closure, accumulated neurons, and frequent perinatal death. Design accordingly: conditional Bak1 flox alleles combined with Bax flox and a tissue-restricted Cre for apoptosis-resistance studies, or groove mutants when distinguishing activation from effector pore formation during BH3 mimetic pharmacology.
Global Bak1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Bak1 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Bak1 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Bak1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Bak1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Bak1 Knockout mouse models are available?
When Bak1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Bak1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Bak1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Bak1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Bak1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.