Bax Knockout mouse models
Mouse Bax, ortholog of human BAX, encodes the cytosolic counterpart to BAK and executes mitochondrial outer membrane permeabilization once activated by upstream ligands. In healthy cells BAX cycles between cytosol and mitochondria, its alpha9 helix sequestered in its own hydrophobic groove and continuously retrotranslocated by BCL2L1 and MCL1, so commitment requires BH3-only activators (BID, BIM, PUMA) to engage either the canonical groove or the alpha1 to alpha6 rear site, driving conformational opening, membrane insertion, symmetric BH3-in-groove dimers, and pore formation upstream of APAF1 apoptosome assembly. Since BAK1 is largely redundant, informative genetics almost always requires compound alleles, and germline Bax Bak1 double nulls are frequently perinatal lethal with retained interdigital webs and expanded neural progenitors. Conditional Bax flox stocks paired with Bak1 flox and a lineage-restricted Cre are therefore the practical route, with non-functional point knockins reserved for questions about oligomerization mechanism rather than absolute apoptotic capacity.
Whole body loss of Bax gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Bax Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Bax knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Bax-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250396 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Bax Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Bax allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Bax Knockout mouse models are available?
When Bax Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Bax knockout embryonic lethal in mice?
It depends on background and allele design. Some Bax germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Bax animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Bax?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.