Bbc3 Knockout mouse models
Bbc3, ortholog of human BBC3, encodes PUMA, a BH3-only protein that is among the most direct transcriptional outputs of p53 and therefore the principal transducer of DNA damage, oncogene stress, and glucocorticoid or cytokine withdrawal into mitochondrial apoptosis. PUMA is unusual among BH3-only members in binding all antiapoptotic relatives, BCL2, BCL2L1, BCL2L2, MCL1, and BCL2A1, with high affinity, so it both liberates and directly activates BAX and BAK; p53-independent induction via FOXO3A and CHOP extends its reach to trophic factor loss and ER stress. Nulls are viable and fertile but show marked resistance of thymocytes and hematopoietic progenitors to irradiation, and phenotypes are frequently blunted because Pmaip1 (NOXA), Bid, and Bcl2l11 cover overlapping stress inputs, making compound alleles necessary. Choose a conditional null when asking whether a tissue requires PUMA for damage-induced deletion, and pair it with p53 pathway alleles when tumor latency or therapy response is the endpoint.
Bbc3 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Bbc3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Bbc3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Bbc3-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 201425 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Bbc3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Bbc3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Bbc3 Knockout mouse models are available?
When Bbc3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Bbc3 knockout embryonic lethal in mice?
It depends on background and allele design. Some Bbc3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Bbc3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Bbc3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.