Bcl2 Knockout mouse models
Bcl2 is the mouse ortholog of human BCL2 and encodes the founding antiapoptotic guardian of the mitochondrial pathway, a tail-anchored protein whose BH1, BH2, and BH3 helices form an elongated hydrophobic groove that captures the BH3 helices of activators and effectors, restraining BAX and BAK before permeabilization. Its oncogenic biology is dosage driven rather than mutational: the human t(14;18) translocation places BCL2 under immunoglobulin heavy chain control in follicular lymphoma, best recapitulated in mice by Emu-driven or conditionally activated overexpression rather than a point mutant. Nulls survive birth but develop polycystic kidneys, lymphoid involution, and hair greying. Because Bcl2l1, Mcl1, and Bcl2l2 buffer loss in most tissues, single deletion often looks silent. Epitope-matched humanization is the decisive design when venetoclax pharmacology is the endpoint, including engineered Gly101Val resistance, while conditional nulls and inducible overexpression alleles answer survival dependency and lymphomagenesis questions respectively.
Global Bcl2 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Bcl2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Bcl2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Bcl2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 231361 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Bcl2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Bcl2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Bcl2 Knockout mouse models are available?
When Bcl2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Bcl2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Bcl2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Bcl2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Bcl2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.