Brca1 Humanized Mouse
Since 1998, ingenious targeting laboratory has delivered 2,800+ projects with 100% germline transmission guarantee and 800+ peer reviewed publications.
Brca1 is a focus locus for humanized mouse models. ingenious targeting laboratory designs and delivers the line to your study specification with ES cell based targeting and documented milestones.
Get a Quote in 24 HoursScientific design
Constitutive null alleles of Brca1 are embryonic lethal in published IMPC data. A conditional knockout strategy preserves a floxed allele for tissue or temporal control while avoiding global loss.
6 existing alleles are indexed for Brca1, including 1 conditional. Where the published set lacks the modification you need, ingenious targeting laboratory builds that allele with documented germline transmission.
Humanization replaces mouse coding sequence with human ortholog sequence at the endogenous locus, maintaining native regulatory context. ES cell targeting documents junctions and expression before germline transmission.
Human ortholog BRCA1 guides humanization design so human coding sequence replaces the mouse exons under endogenous control.
Quotes return in about 24 hours. Study ready cohorts often arrive near 26 weeks from contract start when breeding is direct.
What researchers say
“ingenious targeting laboratory delivered exactly the floxed allele we designed, with clear milestones and germline confirmed founders.”
“The team translated our humanization requirements into a knockin strategy we could breed and phenotype on schedule.”
“Clear communication on allele design and germline transmission made our conditional knockout program straightforward to execute.”
Selected publications
- Microglia alter sex-specific cerebellar myelination following placental hormone loss
- Impact of absent in melanoma 2 on head and neck squamous cell carcinoma development
- CLNS1A regulates genome stability and cell cycle progression to control CD4 T cell function and autoimmunity
FAQ
How long does a Brca1 humanized project take?
Most custom humanized projects run near twenty six weeks from contract activation to study ready animals when breeding is straightforward. Complex humanization or multi allele stacks can add time. We return detailed quotes within about twenty four hours so you can align cohort start dates with grant or IND milestones.
Is Brca1 knockout embryonic lethal in mice?
Lethality depends on genetic background and exact allele design. Some Brca1 germline knockouts are viable, others require conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null approaches before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak patterns. We map your organ and cell type to a short list of proven Cre lines, then discuss reporter crosses and controls. We prioritize drivers with strong community validation for your tissue.
Do you ship live Brca1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a custom project with cryo or live dispatch options depending on cohort timing and geography.
How do I request a quote for Brca1?
Use the catalog inquire buttons or the request quote form with your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.