C3 Knockout mouse models
C3, ortholog of human C3, encodes the pivotal complement component whose proteolytic activation by either the classical and lectin pathway C4b2a convertase or the alternative pathway C3bBb releases the C3a anaphylatoxin and exposes a reactive thioester in the C3b thioester-containing domain that covalently tags surfaces. Deposited C3b seeds further convertase assembly for amplification, is degraded by factor I to iC3b and C3dg for CR3, CR4, and CR2 dependent phagocytosis and B cell priming, and feeds C5 convertase formation. Loss produces pyogenic infection susceptibility, impaired immune complex clearance, and blunted antibody responses, while dysregulated turnover underlies C3 glomerulopathy and paroxysmal nocturnal hemoglobinuria pathology. No paralog compensates, making the null definitive. Humanization is the design of record for compstatin family peptides and pegcetacoplan, which bind human C3 and do not engage the mouse protein, whereas hepatocyte-conditional deletion resolves liver-derived versus local myeloid C3 contributions.
C3 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What C3 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct C3 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| C3-KO | Knockout | KO/CKO mice | live | KO 191193 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The C3 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a C3 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What C3 Knockout mouse models are available?
When C3 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is C3 knockout embryonic lethal in mice?
It depends on background and allele design. Some C3 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live C3 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for C3?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.