C5ar1 Conditional Knockout mouse models
C5ar1 encodes C5aR1 (CD88), the mouse ortholog of human C5AR1, a Galphai-coupled seven-transmembrane receptor that engages the carboxy-terminal region and core of C5a through a two-site mechanism involving its acidic N-terminal segment and transmembrane pocket, then signals via PI3Kgamma, PLCbeta, and beta-arrestin to drive neutrophil chemotaxis, oxidative burst, granule release, tissue factor induction, and NLRP3 priming. Output is shaped by C5a desArg generated by carboxypeptidase N and by the decoy-like second receptor C5ar2, which lacks G protein coupling but modulates beta-arrestin dependent signaling; that pairing means C5ar1 deletion alone may not fully reproduce pharmacological blockade. Nulls are viable and protected in sepsis, ANCA vasculitis, and ischemia reperfusion models, the same axis targeted by avacopan. Choose conditional alleles with myeloid or endothelial Cre when cell of action is the question, and humanized knockins when antagonist potency or species-restricted binding governs the readout.
Conditional deletion of C5ar1 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to C5ar1 floxed stock yields usable cohorts.
What C5ar1 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct C5ar1 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| C5ar1-Flox | Conditional Knockout | KO/CKO mice | live | CKO 200282 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The C5ar1 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a C5ar1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What C5ar1 Conditional Knockout mouse models are available?
When C5ar1 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is C5ar1 knockout embryonic lethal in mice?
It depends on background and allele design. Some C5ar1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live C5ar1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for C5ar1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.