Cacna1a Knockin mouse models
Cacna1a is the mouse ortholog of human CACNA1A and encodes CaV2.1, the pore-forming α1A subunit of presynaptic P/Q-type calcium channels, built from four homologous repeats each contributing an S4 voltage sensor and a P-loop to the selectivity filter, with a cytoplasmic synprint motif that tethers syntaxin-1A and SNAP-25 for tight coupling of calcium entry to vesicle fusion. Three distinct human mutation classes map to different alleles: truncating loss of function causes episodic ataxia type 2, gain-of-function substitutions such as R192Q and S218L cause familial hemiplegic migraine type 1, and a C-terminal polyglutamine expansion causes spinocerebellar ataxia type 6. Constitutive nulls develop severe ataxia and dystonia and die around weaning, so cerebellar conditional deletion using Pcp2-Cre or granule cell drivers is required for adult physiology. CaV2.2 partially substitutes at some central terminals, so record paired-pulse and calcium imaging readouts rather than relying on transmitter release amplitude alone.
An engineered Cacna1a allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Cacna1a Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Cacna1a knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cacna1a-E1980A | Knockin | point mutantion mice | embryo cryopreservation | KI 200279 | Inquire |
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Cacna1a Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cacna1a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cacna1a Knockin mouse models are available?
When Cacna1a Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cacna1a knockout embryonic lethal in mice?
It depends on background and allele design. Some Cacna1a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cacna1a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cacna1a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.