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Cacna1b Conditional Knockout mouse models
Encoding CaV2.2, the α1B pore subunit of N-type calcium channels, Cacna1b is the mouse ortholog of human CACNA1B and dominates transmitter release at nociceptive primary afferent terminals and sympathetic neurons, where it is inhibited voltage-dependently by Gβγ binding to the I-II linker downstream of opioid and GABA-B receptors and trafficked in complex with CRMP2 and collapsin response signaling. Mutually exclusive splicing of exon 37a versus 37b creates a dorsal root ganglion variant with enhanced tonic G protein modulation, and the channel is the target of the conopeptide ziconotide. Because global nulls are viable but hypoalgesic in neuropathic and inflammatory assays, the informative fork is a conditional null crossed to Advillin-Cre or Scn10a-Cre to establish afferent-specific contribution versus humanization of the outer pore and drug-binding regions for species-selective blocker pharmacology. CaV2.1 upregulates at central synapses after deletion, so include compensation controls when interpreting spinal transmission endpoints.
Tissue restricted knockout of Cacna1b keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Cacna1b Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cacna1b conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cacna1b-Flox | CKO | CKO | DEVELOPING | CKO 264112 | Inquire |
| These mice carry loxP sites flanking exon5-6 of Cacna1b gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Cacna1b gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Cacna1b Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cacna1b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cacna1b Conditional Knockout mouse models are available?
When Cacna1b Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cacna1b knockout embryonic lethal in mice?
It depends on background and allele design. Some Cacna1b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cacna1b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cacna1b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the Cacna1b Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.