Casp9 Conditional Knockout mouse models
Casp9, ortholog of human CASP9, encodes the initiator caspase of the intrinsic pathway, whose CARD docks onto the CARD ring of APAF1 after cytochrome c and dATP driven apoptosome assembly, converting the monomeric zymogen into a holoenzyme that cleaves and activates caspases 3 and 7. Activity is set by dimerization rather than proteolysis alone and is restrained by XIAP BIR3 binding and by inhibitory phosphorylation from AKT and ERK, so apoptosome stoichiometry and IAP tone both shape the threshold. Germline nulls show markedly reduced neural progenitor apoptosis, exencephaly, and perinatal lethality on sensitive backgrounds, mirroring Apaf1 and Casp3 deficiency and placing the axis firmly in brain development. No other caspase substitutes for apoptosome coupling, so redundancy is minimal. Conditional flox alleles with lineage-restricted Cre are the practical route past lethality, while catalytically dead knockins or CARD mutants answer whether a phenotype requires proteolysis or merely platform occupancy.
Conditional deletion of Casp9 limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Casp9 floxed stock yields usable cohorts.
What Casp9 Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Casp9 conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Casp9-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 2100009 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Casp9 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Casp9 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Casp9 Conditional Knockout mouse models are available?
When Casp9 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Casp9 knockout embryonic lethal in mice?
It depends on background and allele design. Some Casp9 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Casp9 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Casp9?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.