Cd19 Conditional Knockout mouse — B cell specific context
Cd19, ortholog of human CD19, encodes a B lineage immunoglobulin superfamily coreceptor with two extracellular Ig-like domains that partners with Cr2, Cd81 and Cd225 to lower the B cell receptor activation threshold, recruiting PI3K to phosphorylated cytoplasmic tyrosines and amplifying Btk and Akt signaling. Its dominant translational role is as the antigen for CD19-directed CAR T cells and for blinatumomab, and because those biologics recognize human epitopes that murine Cd19 does not present, an epitope-matched humanized CD19 knockin is the only credible platform for efficacy, B cell aplasia and cytokine release studies. A conventional null instead answers a developmental question, yielding reduced marginal zone and B1 populations with blunted humoral responses. Investigators should also note that Cd19-Cre is a knockin driver, so the Cre allele itself is a functional null and heterozygosity must be controlled. Pair alleles with flow cytometric B cell subset analysis, antigen-specific titers, and CAR persistence readouts.
A floxed Cd19 allele paired with Cre gives spatial control that whole body knockouts cannot offer. People use it to separate developmental roles from adult homeostasis, to model somatic mutations, and to match disease that begins in one organ. For B cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on b-cell while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Cd19-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 230184 | Inquire |
Pricing and quotes
The Cd19 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cd19 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Cd19 Conditional Knockout mouse models are available?
When Cd19 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cd19 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cd19 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for b cell specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to b cell specific, so we start driver selection there.
Do you ship live Cd19 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cd19?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.