Cd14 Conditional Knockout mouse — B cell specific context
Cd14 encodes the mouse ortholog of human CD14, a leucine-rich repeat horseshoe glycoprotein present both as a GPI-anchored surface receptor on monocytes, macrophages, and neutrophils and as a soluble plasma form generated by shedding or direct secretion. Its function is ligand delivery rather than signaling: CD14 accepts lipopolysaccharide extracted from bacterial membranes by LBP and loads lipid A into the TLR4 and MD-2 (Ly96) complex, enabling both MyD88 dependent surface responses and CD14 dependent endocytosis that licenses TRIF and type I interferon output, and it similarly assists TLR2 lipopeptide recognition and nucleic acid sensing TLR trafficking. Nulls are viable and profoundly hyporesponsive to low-dose LPS while retaining responses to high-dose stimulation, a dose-dependent phenotype that must be built into endotoxemia study design. Humanization is correct for anti-CD14 antibody and sepsis therapeutic programs, whereas conditional deletion with myeloid or hepatocyte Cre partitions membrane and soluble CD14 contributions.
Cd14 conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype. For B cell work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on b-cell while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Cd14-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 231269 | Inquire |
Pricing and quotes
The Cd14 Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cd14 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Cd14 Conditional Knockout mouse models are available?
When Cd14 Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cd14 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cd14 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for b cell specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to b cell specific, so we start driver selection there.
Do you ship live Cd14 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cd14?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.