Cd274 Knockin mouse models
Cd274 encodes PD-L1 (B7-H1), the mouse ortholog of human CD274, a type I membrane protein with IgV and IgC2 domains whose IgV face engages PD-1 (Pdcd1) to recruit SHP-2 to the PD-1 ITSM, dephosphorylating CD28 and proximal TCR components and thereby raising the activation threshold of effector T cells. Surface abundance is controlled transcriptionally by IFN-gamma through JAK and STAT1 to IRF1, by MYC and NF-kappaB, by structural variants of the 3 prime untranslated region, and post-translationally by CMTM6 mediated recycling versus glycosylation-dependent degradation; PD-L1 also binds CD80 in cis and exists in exosomal and soluble forms. Nulls are viable but show exaggerated T cell responses and autoimmune susceptibility. Because atezolizumab and durvalumab do not cross-react, epitope-matched humanization is the decisive allele for checkpoint pharmacology and biomarker work, whereas conditional deletion with tumor cell, dendritic cell, or endothelial Cre resolves which compartment enforces suppression.
An engineered Cd274 allele can introduce a human coding region, a point change, or a fluorescent reporter without random transgene integration noise.
What Cd274 Knockin mouse models are available?
ingenious targeting laboratory offers 1 distinct Cd274 (PD-L1) knockin catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cd274-2A-Dre | Knockin | Cre/Dre Toolbox of Mice | sperm cryopreservation | KI 242195 | Inquire |
Designing a Cd274 Knockin allele
Knockin is the way to attack regulation rather than presence, which is where most of the unanswered biology sits. A fluorescent or luciferase reporter placed in frame at the locus lets you watch interferon gamma driven induction in real time across tumor, stroma and draining node, something antibody staining after tissue dissociation cannot provide. Deleting the IRF1 binding element in the regulatory region yields an allele that retains basal expression but cannot be induced, separating constitutive from inflammation-driven suppression. Truncating the three prime untranslated region reproduces the structural variants that stabilize the transcript in human lymphoma and gives a genetic overexpression model at endogenous copy number. Finally, substitutions on the face that contacts CD80 while sparing the PD-1 interface separate cis inhibition from trans signaling. Read out surface protein by flow, transcript stability by actinomycin chase, and T cell function in co-culture and in tumor-bearing animals.
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Cd274 Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cd274 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cd274 Knockin mouse models are available?
When Cd274 Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cd274 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cd274 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cd274 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cd274?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.