Cd28 Knockout mouse models
Mouse Cd28, ortholog of human CD28, encodes the dominant costimulatory receptor of naive T cells and forms a disulfide-linked homodimer whose single IgV domain uses the MYPPPY motif to bind CD80 and CD86. Ligation clusters CD28 in the immunological synapse where its cytoplasmic YMNM motif recruits PI3K p85 and GRB2 while a proline-rich PYAP motif binds LCK and ITK, amplifying PLCgamma1, NF-kappaB, and NFAT signaling to drive IL-2 transcription, BCL2L1 induction, glucose uptake, and clonal expansion, and to sustain regulatory T cell homeostasis. Nulls are viable with impaired T-dependent antibody responses, reduced germinal centers, and Treg deficits. CTLA4 competes for the same ligands but antagonizes rather than substitutes, so no true redundancy exists. Humanized CD28 knockins are essential for CD28 bispecific and superagonist safety work given the TGN1412 precedent, while conditional alleles with Cd4-Cre or Foxp3-Cre separate conventional from regulatory T cell requirements.
Whole body loss of Cd28 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Cd28 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cd28 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cd28-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 190034 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cd28 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cd28 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cd28 Knockout mouse models are available?
When Cd28 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cd28 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cd28 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cd28 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cd28?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.