Conventional / global / constitutive KO
●2 Models Available
READY TO SHIP!
SPERM · EMBRYO
Cd28 Humanized, Knockout mouse models
Browse 2 Cd28 mouse models including humanized, knockout variants from ingenious targeting laboratory. Available as Cd28 humanized mouse, Cd28 knockout mouse. Contact us for availability and fast turnaround.
Why model Cd28
Mouse Cd28, ortholog of human CD28, encodes the dominant costimulatory receptor of naive T cells and forms a disulfide-linked homodimer whose single IgV domain uses the MYPPPY motif to bind CD80 and CD86. Ligation clusters CD28 in the immunological synapse where its cytoplasmic YMNM motif recruits PI3K p85 and GRB2 while a proline-rich PYAP motif binds LCK and ITK, amplifying PLCgamma1, NF-kappaB, and NFAT signaling to drive IL-2 transcription, BCL2L1 induction, glucose uptake, and clonal expansion, and to sustain regulatory T cell homeostasis. Nulls are viable with impaired T-dependent antibody responses, reduced germinal centers, and Treg deficits. CTLA4 competes for the same ligands but antagonizes rather than substitutes, so no true redundancy exists. Humanized CD28 knockins are essential for CD28 bispecific and superagonist safety work given the TGN1412 precedent, while conditional alleles with Cd4-Cre or Foxp3-Cre separate conventional from regulatory T cell requirements.
What Cd28 mouse models are available?
ingenious targeting laboratory offers 2 distinct Cd28 (CD28) catalog mouse models, featuring conditional knockout, standard knockout, and humanized. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
| Model Availability | Allele Class Options |
|---|---|
| 2 Ready Catalog Lines | Conditional Knockout (cKO) |
| Custom Model Generation | Constitutive Knockout (KO) |
| Advanced Modifications | Humanized, Knockin, Transgenic |
- Quality Control
- Documented Germline Transmission
- Quality Control
- Strict United States QC Guidelines
- Quality Control
- Cryo or Live Dispatch Options
Cd28 model types
PathAvailability
Knockout
Conditional knockout
Knockin
Humanized
Transgenic / overexpression
Prefer the order form? Order. Need an allele that is not listed? Request a quote.
Cd28 allele types
Knockout, conditional, knockin, humanized, and related paths for Cd28 (human CD28). Catalog lines ship when inventory exists. Everything else is a generation quote.
Knockout
Conditional KO
Inducible conditional KO
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI
Tag KI
Conditional KI
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Targeted Transgenic
Safe harbor
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre
Tamoxifen or dox inducible Cre
Dual recombinase
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Gene specific reporter knockin
Standalone reporter catalog
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Available Cd28 catalog models
2 Cd28 lines with QC documentation and technical support. Cd28 humanized models carry the human gene sequence for translational research.
Looking for a Cd28 mouse model?
We have 2 Cd28 models available — including Humanized, Knockout types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.
Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.
Order catalog model2
Models Available
2
Model Types
24 hrs
Quote Turnaround
Included
QC Documentation
About Cd28 Mouse Model Types
A Cd28 knockout mouse has the Cd28 gene permanently inactivated, enabling loss of function studies. A Cd28 humanized mouse replaces the mouse Cd28 gene with the human ortholog for translational research and drug development.
Modifications available
Jump to catalog backed pages for Cd28 organized by modification type. Each URL is indexable and matches common search patterns.
Cd28 mouse model FAQ
What Cd28 Humanized mouse models are available?
When Cd28 Humanized lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cd28 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cd28 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cd28 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cd28?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ingenious targeting laboratory
Send the Cd28 line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.