Cdkn2a Knockin mouse models
Cdkn2a, the mouse ortholog of human CDKN2A, is unusual because a single locus encodes two unrelated tumor suppressors from alternative reading frames: p16INK4a, an ankyrin repeat protein that allosterically inhibits CDK4 and CDK6 to keep RB hypophosphorylated, and p19ARF (human p14ARF) from an alternate first exon, which sequesters MDM2 in the nucleolus to stabilize p53. Deletion of the entire locus therefore disables the RB and p53 axes simultaneously, which is why bulk 9p21 loss is among the most common events in melanoma, glioma, pancreatic cancer and mesothelioma but is uninterpretable for mechanism. Allele design must be isoform-specific: exon 1 alpha targeted alleles remove p16 alone, exon 1 beta targeted alleles remove ARF alone, and only the combined deletion mimics the human co-deletion. Cdkn2b lies adjacent, is frequently co-deleted, and p15 partially compensates for p16 loss. Match allele to endpoint, using p16-specific alleles for CDK4/6 inhibitor sensitivity and ARF-specific alleles for p53 pathway questions.
Cdkn2a knockin models place a defined sequence at the endogenous locus. Expression stays under native regulation, which matters for reporters, tags, and precise allele swaps.
What Cdkn2a Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Cdkn2a knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Cdkn2a Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cdkn2a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cdkn2a Knockin mouse models are available?
When Cdkn2a Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cdkn2a knockout embryonic lethal in mice?
It depends on background and allele design. Some Cdkn2a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cdkn2a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cdkn2a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.