Cfi Knockout mouse models
Cfi, ortholog of human CFI, encodes complement factor I, the sole protease that irreversibly inactivates deposited C3b and C4b. The secreted two-chain enzyme couples a heavy chain of FIMAC, SRCR, and two LDL receptor class A modules to a chymotrypsin-like serine protease light chain that is unusual in requiring an allosteric partner: catalysis proceeds only when C3b or C4b is engaged with a cofactor such as factor H, membrane cofactor protein CD46, C4b-binding protein, or CR1, which explains both its exceptional specificity and its sequential cleavage of C3b at two arginine sites to yield iC3b and then C3dg. Human deficiency causes secondary C3 depletion with pyogenic infection, and heterozygous mutations predispose to atypical hemolytic uremic syndrome and macular degeneration. Nulls consume C3 spontaneously and lack compensating proteases. Deploy the null for uncontrolled amplification phenotypes, hepatocyte-conditional alleles to test liver source, and humanization when factor I supplementation or cofactor-dependent activity is measured.
A conventional Cfi knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Cfi Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cfi knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cfi-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 200117 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cfi Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cfi allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cfi Knockout mouse models are available?
When Cfi Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cfi knockout embryonic lethal in mice?
It depends on background and allele design. Some Cfi germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cfi animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cfi?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.