Cflar Knockout mouse models
Cflar encodes cellular FLICE inhibitory protein, the mouse ortholog of human CFLAR, a death effector domain protein recruited to FADD within death-inducing signaling complexes where it heterodimerizes with procaspase-8. The long isoform retains a catalytically dead caspase-like domain that paradoxically stabilizes partial caspase-8 activity sufficient for RIPK1 and CYLD cleavage while blocking full processing and apoptosis, whereas short isoforms truncate after the death effector domains and inhibit outright; abundance is set by NF-kappaB dependent transcription and rapid ITCH and cullin-3 mediated turnover, making CFLAR a labile rheostat between survival, apoptosis, and necroptosis. Germline deletion is embryonic lethal around E10.5 with cardiac and vascular failure, phenocopying Casp8 and Fadd loss, and is rescued by concurrent Ripk3 or Mlkl deficiency. Conditional flox alleles with keratinocyte, intestinal epithelial, or myeloid Cre are therefore required, and isoform-selective knockins are the tool of choice for separating long from short isoform function.
A conventional Cflar knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Cflar Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Cflar knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cflar-KO | Knockout | KO/CKO mice | In production — inquire | KO 252922 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Cflar Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cflar allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cflar Knockout mouse models are available?
When Cflar Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cflar knockout embryonic lethal in mice?
It depends on background and allele design. Some Cflar germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cflar animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cflar?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.