Chek2 Knockout mouse models
Chek2, mouse ortholog of human CHEK2, encodes CHK2, an ATM effector kinase built from an SQ/TQ cluster, an FHA phosphopeptide binding domain and a kinase domain, activated when ATM phosphorylates threonine 68 to promote FHA-mediated dimerization and trans-autophosphorylation at the activation loop. Active CHK2 phosphorylates p53 at serine 20 to disrupt MDM2 binding, targets CDC25A and CDC25C for degradation or 14-3-3 sequestration, and phosphorylates BRCA1 and E2F1, enforcing G1 arrest and apoptosis after double strand breaks. The truncating 1100delC and missense I157T alleles confer moderate breast and prostate cancer risk, making patient-variant knockins the appropriate tool for penetrance and variant reclassification studies. Null mice are viable and radioresistant in thymocytes with defective p53 stabilization, so constitutive alleles are entirely workable, unlike Chek1. Because CHK2 acts on breaks and CHK1 on replication stress, they are not redundant. Choose nulls for apoptotic threshold and radiation response, and knockins for allele-specific cancer risk modeling.
Whole body loss of Chek2 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Chek2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Chek2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Chek2-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 2107927 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Chek2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Chek2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Chek2 Knockout mouse models are available?
When Chek2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Chek2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Chek2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Chek2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Chek2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.