Chrd Knockout mouse models
Chrd, the mouse ortholog of human CHRD, encodes chordin, a secreted protein built from four cysteine-rich CR domains that binds BMP2 and BMP4 with high affinity and blocks receptor engagement, establishing the extracellular gradient that patterns dorsoventral axis, pharyngeal apparatus, and inner ear. Its inhibitory activity is itself regulated proteolytically: BMP1 and the tolloid proteases TLL1 and TLL2 cleave chordin to release active ligand, while twisted gastrulation encoded by Twsg1 forms ternary complexes that can either strengthen or relieve inhibition depending on cleavage state. Nulls show strain-dependent pharyngeal, cardiovascular, and ear defects resembling DiGeorge phenotypes with variable perinatal lethality, so background control is as consequential as allele design. Domain-specific deletions removing individual CR modules or cleavage-site knockins are more informative than complete ablation when the question is gradient shape rather than presence. Overlapping antagonism from Nog, Grem1, and Cer1 means compound alleles are needed to fully derepress BMP signaling at a given endpoint.
Whole body loss of Chrd gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Chrd Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Chrd knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Chrd-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 201252 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Chrd Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Chrd allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Chrd Knockout mouse models are available?
When Chrd Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Chrd knockout embryonic lethal in mice?
It depends on background and allele design. Some Chrd germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Chrd animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Chrd?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.