Ctla4 Knockout mouse models
Ctla4, ortholog of human CTLA4, encodes CD152, an inhibitory CD28 family homodimer that shares the MYPPPY ligand motif but binds CD80 and CD86 with far higher avidity, outcompeting CD28 and physically stripping ligands from antigen presenting cells by transendocytosis, a cell-extrinsic mechanism central to regulatory T cell suppression. Surface residence is brief because the cytoplasmic YVKM motif recruits AP-2 for constitutive clathrin-mediated internalization and LRBA-dependent recycling, and the same motif engages PP2A and SHP-2. Germline deletion is catastrophic, with fatal lymphoproliferation, myocarditis, and pancreatitis by three to four weeks, so adult studies demand conditional flox alleles with Foxp3-Cre or Cd4-CreER to separate regulatory from conventional T cell requirements. No paralog compensates, since CD28 is the functional antagonist rather than a substitute. Humanized CTLA4 knockins are essential for ipilimumab and Fc-engineered antibody work, and haploinsufficient alleles suit human CTLA4 insufficiency and LRBA deficiency biology.
A conventional Ctla4 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Ctla4 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Ctla4 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Ctla4-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 202037 | Inquire |
Designing a Ctla4 Knockout allele
The straight null is not a tool for chronic experiments, it is a short and violent phenotype: multiorgan lymphoproliferation, myocarditis, pancreatitis, and death within three to four weeks, so readouts must be collected in a narrow window before wasting confounds them. Deleting the exons encoding the ectodomain and transmembrane segment gives an unambiguous protein null rather than a truncated species that could still dimerize. Heterozygotes are more useful for translational work, since reduced dosage models human CTLA4 insufficiency with variable lymphocytic infiltration and incomplete penetrance that itself demands large cohorts. Kinetics shift with strain, so fix background and use littermate controls before comparing groups. Informative endpoints are survival, weight curves, expansion of CD44 high CD62L low populations, autoantibody titers, and blinded histopathology across heart, pancreas, lung and salivary gland rather than scoring one tissue.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ctla4 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ctla4 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ctla4 Knockout mouse models are available?
When Ctla4 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ctla4 knockout embryonic lethal in mice?
It depends on background and allele design. Some Ctla4 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ctla4 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ctla4?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.