Ctsb Knockout mouse models
Mouse Ctsb, ortholog of human CTSB, encodes cathepsin B, a papain-family lysosomal cysteine protease distinguished by an occluding loop that folds over the primed subsites, conferring carboxydipeptidase activity in addition to endopeptidase function. The proenzyme is trafficked by mannose-6-phosphate receptors and activated at acidic pH by autocatalysis or by cathepsin D and legumain, and its substrates span bulk lysosomal turnover, maturation of other proenzymes, extracellular matrix components after secretion, and inflammatory mediators, with lysosomal leakage of cathepsin B implicated in NLRP3 inflammasome activation, tumor invasion, and necrotic death. Nulls are viable and fertile, partly because cathepsins L, Z, and S share substrate space, so meaningful phenotypes often require compound Ctsb Ctsl or Ctsb Ctsz alleles. Use conditional deletion with myeloid or tumor cell Cre when localizing the relevant protease pool, and active site or occluding loop knockins when endopeptidase versus exopeptidase activity is the mechanistic question.
Ctsb null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Ctsb Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct Ctsb knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Ctsb Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Ctsb allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Ctsb Knockout mouse models are available?
When Ctsb Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Ctsb knockout embryonic lethal in mice?
It depends on background and allele design. Some Ctsb germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Ctsb animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Ctsb?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.