Dhh Knockin mouse models
Dhh, mouse ortholog of human DHH, encodes Desert hedgehog, an autoprocessing morphogen that undergoes intramolecular cleavage with cholesterol modification and palmitoylation before binding PTCH1 to relieve inhibition of SMO, stabilizing GLI2 and GLI3 for target transcription. Unlike the broadly expressed SHH, its expression is restricted chiefly to Sertoli cells, where it acts on peritubular and Leydig progenitors to drive steroidogenic differentiation, and to Schwann cells, where it builds the perineurium and nerve-blood barrier. Human biallelic mutations cause 46,XY gonadal dysgenesis with or without minifascicular polyneuropathy, a phenotype well matched by patient-derived missense knockins that impair processing or receptor binding. Mouse nulls are viable but males show Leydig hypoplasia, infertility, and abnormal nerve sheaths, so constitutive deletion is workable while conditional floxed alleles with Amh-Cre or Dhh-Cre separate testicular from peripheral nerve requirements. Because Shh and Ihh signal through the same PTCH1 and SMO machinery, interpret pathway-level readouts with paralog expression documented for the tissue scored.
Knockin designs at Dhh support head to head comparisons between wild type and modified alleles because regulatory DNA stays in place.
What Dhh Knockin mouse models are available?
ingenious targeting laboratory offers 2 distinct Dhh knockin catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Why this approach
Knockin and humanized formats keep regulatory context at the endogenous locus. For your target tissue focused programs, that matters when expression timing, splice isoforms, or allele dosage drive biology. Random integration transgenics can still help, but targeted alleles usually give cleaner pharmacology readouts.
Pricing and quotes
The Dhh Knockin lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dhh allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dhh Knockin mouse models are available?
When Dhh Knockin lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dhh knockout embryonic lethal in mice?
It depends on background and allele design. Some Dhh germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dhh animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dhh?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.