Dkk1 Knockout mouse models
Dkk1, the mouse ortholog of human DKK1, encodes a secreted glycoprotein with two cysteine rich domains that antagonizes canonical Wnt signaling by binding the third and fourth beta-propeller modules of LRP5 and LRP6 with high affinity, blocking Wnt induced LRP6 signalosome assembly with Frizzled, and in some contexts cooperating with Kremen1 and Kremen2 to internalize the coreceptor. Its function is strictly extracellular and dose dependent, which is why heterozygotes show measurable phenotype and why DKK1 neutralizing antibodies are tested in myeloma bone disease and osteoporosis. Germline null embryos die at birth lacking anterior head structures and with limb malformation because DKK1 is required for head organizer activity, so conditional deletion is necessary for adult bone and hematopoietic questions. Dkk2 and Dkk4 bind the same LRP sites, with DKK2 acting as agonist or antagonist depending on Kremen levels, so compound alleles matter. Use conditional nulls for tissue Wnt tone and humanized knockins when testing antibody epitope engagement.
Global Dkk1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Dkk1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dkk1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dkk1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 250400 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Dkk1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dkk1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dkk1 Knockout mouse models are available?
When Dkk1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dkk1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Dkk1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dkk1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dkk1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.