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Dlat Conditional Knockout mouse models
Dlat is the mouse ortholog of human DLAT and encodes the E2 dihydrolipoamide S-acetyltransferase that forms the trimeric 60-subunit core of the pyruvate dehydrogenase complex, presenting lipoyl domains that swing acetyl groups from E1 to coenzyme A and thereby committing glycolytic pyruvate to acetyl-CoA and the TCA cycle. The lipoyl arm, installed by LIAS and LIPT1, also makes E2 the docking platform for E1, E3 and E3BP, so its loss removes the scaffold on which flux control by Pdk kinases and Pdp phosphatases operates. Complete deficiency produces congenital lactic acidosis, so conditional nulls with Alb-Cre, Ckmm-Cre or Nes-Cre are the practical route for isolating tissue-specific pyruvate oxidation defects, while patient missense knockins retain partial activity and model graded disease. Lipoylated E2 is also the dominant autoantigen of primary biliary cholangitis, making humanized alleles attractive for immunological endpoints; match allele to readout, whether carbon 13 pyruvate tracing, blood lactate, or PDH activity assays.
Tissue restricted knockout of Dlat keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Dlat Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dlat conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dlat-Flox | CKO | CKO | IN PRODUCTION | CKO 260479 | Inquire |
| These mice carry loxP sites flanking exon 5 of Dlat gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Dlat gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Dlat Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dlat allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dlat Conditional Knockout mouse models are available?
When Dlat Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dlat knockout embryonic lethal in mice?
It depends on background and allele design. Some Dlat germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dlat animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dlat?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Breed this line with ITL
Send the Dlat Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.