Dld Knockout mouse models
Mouse Dld, ortholog of human DLD, specifies the FAD-dependent homodimeric E3 dihydrolipoamide dehydrogenase shared by four mitochondrial systems, pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, branched-chain ketoacid dehydrogenase and the glycine cleavage complex, where it reoxidizes reduced lipoamide and passes electrons to NAD+. This shared architecture explains why human DLD mutations cause combined E3 deficiency with simultaneous lactic acidemia, elevated alpha-ketoglutarate, branched-chain ketoaciduria and sometimes hyperglycinemia, and why the enzyme is itself a significant source of reactive oxygen species. Because a global null removes four dehydrogenase activities at once and is not survivable, conditional alleles are the workable design, with hepatocyte, neuronal or cardiomyocyte Cre lines separating hepatic decompensation from encephalopathy and cardiomyopathy. Patient variant knockins, particularly those at the dimer interface, better reproduce the partially active and temperature-sensitive human enzyme. Align allele to endpoint, whether organic acid profiling, complex-resolved dehydrogenase activity assays, or fasting and catabolic stress challenge.
Dld null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Dld Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Dld knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Dld-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 240409 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Dld Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Dld allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Dld Knockout mouse models are available?
When Dld Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Dld knockout embryonic lethal in mice?
It depends on background and allele design. Some Dld germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Dld animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Dld?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.