Egfr Conditional Knockout mouse — lung specific context
Egfr, the mouse ortholog of human EGFR, encodes ERBB1, a receptor tyrosine kinase whose extracellular domains I through IV adopt a tethered autoinhibited conformation until ligand binding to domains I and III exposes the domain II dimerization arm, permitting formation of an asymmetric kinase dimer in which one lobe allosterically activates the other, with autophosphorylated C-terminal tyrosines recruiting GRB2, SHC and PLC gamma to fire RAS-MAPK and PI3K-AKT. Oncogenic exon 19 deletions and L858R destabilize the inactive kinase conformation and confer TKI sensitivity, T790M and C797S confer resistance, and the vIII extracellular truncation drives glioblastoma. Critically, mouse Egfr is not bound by cetuximab and differs in domain III epitopes, so ectodomain humanization is mandatory for antibody and antibody-drug conjugate testing, while inducible mutant knockins model TKI response. Germline nulls die perinatally with strain-dependent severity, so conditional alleles plus a lineage Cre are required for adult skin, intestinal or lung studies.
Egfr conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype. For lung work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Catalog options
Conditional knockout focuses the experiment on lung while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
| Model | Type | Category | Availability | Catalog # | |
|---|---|---|---|---|---|
| Egfr-Flox | Conditional Knockout | KO/CKO mice, disease model mice | embryo cryopreservation | CKO 00133 | Inquire |
Designing a Egfr Conditional Knockout allele
Deleting the receptor is the cleaner genetic maneuver than removing ligands, because seven agonists including Egf, Tgfa, Areg, Hbegf, Ereg, Btc and Epgn converge on it and single ligand nulls are usually silent. Floxing the exons encoding the kinase domain removes catalytic output while avoiding a stable truncated ectodomain that could still dimerize. Choose the driver by tissue: K14-Cre or K5-CreERT2 for epidermis, Villin-CreERT2 for intestinal regeneration after irradiation or dextran sulfate injury, Scgb1a1-CreER or Sftpc-CreERT2 for airway and alveolar epithelium, hGFAP-Cre for glia. Be aware that epidermal deletion produces severe dermatitis with systemic inflammation and weight loss, which contaminates any downstream immune or metabolic readout, so use inducible drivers and short intervals. Score phosphorylated EGFR, ERK and AKT, wound closure or crypt regeneration kinetics, and confirm excision at the protein level rather than by tail genotyping.
Pricing and quotes
The Egfr Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Egfr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with milestones for genotyping, QC, and dispatch.
FAQ
What Egfr Conditional Knockout mouse models are available?
When Egfr Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Egfr knockout embryonic lethal in mice?
It depends on background and allele design. Some Egfr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for lung specific focused experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. Your note points to lung specific, so we start driver selection there.
Do you ship live Egfr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Egfr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.