Egfr Knockout mouse models
Egfr, the mouse ortholog of human EGFR, encodes ERBB1, a receptor tyrosine kinase whose extracellular domains I through IV adopt a tethered autoinhibited conformation until ligand binding to domains I and III exposes the domain II dimerization arm, permitting formation of an asymmetric kinase dimer in which one lobe allosterically activates the other, with autophosphorylated C-terminal tyrosines recruiting GRB2, SHC and PLC gamma to fire RAS-MAPK and PI3K-AKT. Oncogenic exon 19 deletions and L858R destabilize the inactive kinase conformation and confer TKI sensitivity, T790M and C797S confer resistance, and the vIII extracellular truncation drives glioblastoma. Critically, mouse Egfr is not bound by cetuximab and differs in domain III epitopes, so ectodomain humanization is mandatory for antibody and antibody-drug conjugate testing, while inducible mutant knockins model TKI response. Germline nulls die perinatally with strain-dependent severity, so conditional alleles plus a lineage Cre are required for adult skin, intestinal or lung studies.
A conventional Egfr knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Egfr Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Egfr knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Egfr-KO | Knockout | KO/CKO mice, disease model mice | sperm cryopreservation | KO 190268 | Inquire |
Designing a Egfr Knockout allele
A constitutive null is unusually background dependent and that fact should drive the decision. On some strains embryos fail around implantation, on 129 they die in midgestation from placental spongiotrophoblast deficiency, and on outbred or C57BL/6 backgrounds pups survive to birth then die within weeks with immature lung, skin and gut epithelium and progressive neurodegeneration. Any cohort comparison therefore requires a fixed, declared background and littermate controls, and rederivation onto a second strain is often informative in itself. For adult questions the null is the wrong tool; the naturally occurring waved-2 hypomorph, a kinase domain substitution that lowers but does not abolish catalytic activity, is viable and gives graded signaling reduction. Use the null for placental morphometry, lung branching counts, and epidermal barrier assays at defined embryonic and neonatal stages, and confirm complete loss of receptor protein rather than relying on transcript measurement.
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Egfr Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Egfr allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Egfr Knockout mouse models are available?
When Egfr Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Egfr knockout embryonic lethal in mice?
It depends on background and allele design. Some Egfr germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Egfr animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Egfr?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.