Eif4ebp1 Knockout mouse models
Eif4ebp1, mouse ortholog of human EIF4EBP1, encodes 4E-BP1, the principal translational repressor downstream of mTORC1, which binds eIF4E through a canonical YXXXXL phi motif plus a secondary elongated interface and thereby blocks eIF4G recruitment and cap-dependent initiation. RAPTOR docks the TOS motif and mTORC1 phosphorylates a hierarchical series beginning at threonines 37 and 46, then threonine 70 and serine 65, progressively releasing eIF4E and licensing translation of 5 prime TOP and eIF4E-sensitive mRNAs including cyclin D1, MCL1 and MYC. Because 4E-BP1 phosphorylation is rapamycin-resistant relative to S6K1, it is the readout that distinguishes true mTOR kinase inhibitors from rapalogs. Nonphosphorylatable knockin alleles carrying alanine substitutions at the four regulatory sites give constitutive repression and are far more informative than a null for testing whether a tumor depends on cap-dependent translation. Eif4ebp2 and Eif4ebp3 compensate, especially in brain, so compound alleles are needed before concluding requirement.
Eif4ebp1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Eif4ebp1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Eif4ebp1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Eif4ebp1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 191088 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Eif4ebp1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Eif4ebp1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Eif4ebp1 Knockout mouse models are available?
When Eif4ebp1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Eif4ebp1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Eif4ebp1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Eif4ebp1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Eif4ebp1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.