F10 Knockout mouse models
F10 encodes coagulation factor X, the mouse ortholog of human F10 and the convergence point of the intrinsic and extrinsic cascades. The vitamin K dependent zymogen carries an amino-terminal Gla domain whose gamma-carboxyglutamate residues mediate calcium-dependent phospholipid binding, two EGF-like modules, and a trypsin-like protease domain; activation by the tissue factor and factor VIIa complex or by the factor IXa and factor VIIIa intrinsic tenase generates factor Xa, which assembles with factor Va on activated platelet membranes into prothrombinase to convert prothrombin to thrombin, and is checked by antithrombin and tissue factor pathway inhibitor. Complete deficiency is not survivable in mouse, causing embryonic and perinatal lethality from hemorrhage, so hypomorphic or conditional alleles are required for adult phenotyping. Low-expressing knockins that titrate factor X to a few percent of normal are the established route for bleeding and thrombosis endpoints, whereas humanized knockins suit direct oral anticoagulant and reversal agent pharmacology.
Whole body loss of F10 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What F10 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct F10 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F10-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 235011 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The F10 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F10 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F10 Knockout mouse models are available?
When F10 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F10 knockout embryonic lethal in mice?
It depends on background and allele design. Some F10 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F10 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F10?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.