F12 Knockout mouse models
F12 encodes factor XII (Hageman factor), the mouse ortholog of human F12, a zymogen with fibronectin type I and II, EGF, and kringle domains plus a proline-rich region that autoactivates upon contact with polyanionic surfaces including polyphosphate, misfolded protein aggregates, collagen, and artificial materials. Factor XIIa then cleaves prekallikrein to plasma kallikrein, which reciprocally amplifies factor XII activation and liberates bradykinin from high molecular weight kininogen, and it activates factor XI to feed intrinsic coagulation. This dual output makes F12 the entry point for both contact-driven thrombosis and kinin-mediated vascular permeability, with human gain-of-function variants such as Thr309Lys causing hereditary angioedema despite normal C1 inhibitor. Nulls display normal hemostasis yet are protected in arterial thrombosis and stroke models, precisely the dissociation of interest. Knockins of angioedema-associated alleles are the design for permeability and attack-driven endpoints, whereas humanization supports antibody and antisense programs directed at human factor XII.
Whole body loss of F12 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What F12 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct F12 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F12-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200193 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The F12 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F12 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F12 Knockout mouse models are available?
When F12 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F12 knockout embryonic lethal in mice?
It depends on background and allele design. Some F12 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F12 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F12?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.