READY TO SHIP!
DEVELOPING
F13a1 Knockout mouse models
F13a1 is the mouse ortholog of human F13A1 and encodes the catalytic A subunit of factor XIII, a calcium-dependent transglutaminase circulating as an A2B2 heterotetramer and present intracellularly as cytoplasmic A2. Thrombin cleavage of the activation peptide plus calcium-driven dissociation from the B subunit exposes the cysteine active site, which forms gamma-glutamyl-epsilon-lysyl isopeptide crosslinks between fibrin gamma and alpha chains and incorporates alpha-2 antiplasmin, conferring mechanical strength and fibrinolysis resistance on the clot; additional substrates include factor V, von Willebrand factor, and matrix proteins, while macrophage and platelet A subunit contributes to wound healing and phagocytosis. Human A subunit deficiency causes severe bleeding with umbilical hemorrhage and poor healing, and mouse nulls are viable but bleed and show impaired uterine and cardiac repair. The B subunit cannot substitute catalytically. Use nulls or patient knockins for clot stability endpoints and conditional alleles to separate hepatic from myeloid A subunit pools.
F13a1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What F13a1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct F13a1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F13a1-KO | KO | KO | DEVELOPING | KO 262549 | Inquire |
| The exon 7 of F13a1 gene was deleted to generate F13a1 knockout mouse. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The F13a1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F13a1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F13a1 Knockout mouse models are available?
When F13a1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F13a1 knockout embryonic lethal in mice?
It depends on background and allele design. Some F13a1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F13a1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F13a1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the F13a1 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.