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F13b Conditional Knockout mouse models
The carrier B subunit of factor XIII is encoded by mouse F13b, ortholog of human F13B, a plasma glycoprotein composed of ten sushi domains, also called complement control protein modules, that binds the catalytic A2 dimer to form the circulating A2B2 heterotetramer. The B subunit has no transglutaminase activity of its own; its role is to stabilize A2 in plasma, extend its half-life several fold, prevent premature calcium-dependent activation, and modulate presentation of factor XIII to fibrin, dissociating once thrombin cleaves the A subunit activation peptide so catalysis can proceed. Human F13B deficiency therefore produces a secondary reduction in circulating A subunit and a milder bleeding phenotype than A subunit deficiency, an informative dissociation between availability and catalysis. No paralog performs this carrier function. Choose the null when factor XIII stability, clearance, and plasma reservoir biology is the question, hepatocyte-conditional alleles to confirm liver source, and humanization for concentrate replacement pharmacokinetics.
Conditional deletion of F13b limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to F13b floxed stock yields usable cohorts.
What F13b Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct F13b conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F13b-Flox | CKO | CKO | DEVELOPING | CKO 261600 | Inquire |
| These mice carry loxP sites flanking exon1 of F13b gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of F13b gene. | |||||
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The F13b Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F13b allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F13b Conditional Knockout mouse models are available?
When F13b Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F13b knockout embryonic lethal in mice?
It depends on background and allele design. Some F13b germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F13b animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F13b?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Breed this line with ITL
Send the F13b Conditional Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.