F5 Knockout mouse models
Mouse F5 is the ortholog of human F5 and encodes factor V, the nonenzymatic cofactor that raises factor Xa catalytic efficiency toward prothrombin by several orders of magnitude. Synthesized with A1, A2, B, A3, C1, and C2 domains, factor V is activated by thrombin or factor Xa cleavage that releases the large inhibitory B domain, allowing heterodimeric factor Va to bind membrane phosphatidylserine through its C2 discoidin domain and assemble prothrombinase. Downregulation occurs by activated protein C cleavage at Arg306, Arg506, and Arg679, and the human Leiden variant substituting Arg506 with glutamine confers APC resistance and thrombophilia, a mutation class best modeled by homologous knockin rather than null. Complete deficiency is lethal in mouse from embryonic and perinatal hemorrhage, so hypomorphic or conditional alleles are required, while the East Texas splice variant illustrates additional TFPI-related regulatory function. Pair Leiden knockins with thrombosis challenge endpoints and conditional alleles with platelet versus hepatic source questions.
Whole body loss of F5 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What F5 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct F5 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F5-KO | Knockout | KO/CKO mice | live | KO 250956 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The F5 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F5 Knockout mouse models are available?
When F5 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F5 knockout embryonic lethal in mice?
It depends on background and allele design. Some F5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.