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F9 Knockout mouse models
F9, ortholog of human F9, encodes coagulation factor IX and sits on the X chromosome, making hemizygous males the natural genetic configuration for hemophilia B modeling. The vitamin K dependent zymogen presents a Gla domain, two EGF modules, an activation peptide, and a protease domain; cleavage by factor XIa or by the tissue factor and factor VIIa complex yields factor IXa, which assembles with factor VIIIa on activated platelet membranes into intrinsic tenase to activate factor X, with the EGF1 and Gla surfaces mediating cofactor and membrane contacts. Mouse nulls reproduce the coagulopathy and remain the standard preclinical hemophilia B platform. Two allele designs answer different questions: patient-specific missense knockins interrogate residual activity, inhibitor development, and immune tolerance, whereas the hyperfunctional Padua substitution at Arg338 underpins gene therapy potency studies. Humanized F9 knockins are required when human factor IX directed antibody, Fc or albumin fusion pharmacokinetics, or mimetic bispecific activity is the readout.
Global F9 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What F9 Knockout mouse models are available?
ingenious targeting laboratory offers 2 distinct F9 knockout catalog mouse models. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| F9-KO | KO | KO, Disease Model | EMBRYO | KO 18046 | Inquire |
| The F9 gene is located on the X chromosome. In this F9 knockout mouse model, gRNAs were designed targeting Exon8 of F9 gene via CRISPR gene editing technology. Loss of F9 caused coagulopathy in mice. Mice that are homozygous for F9 knockout are viable, fertile and normal in size. Normal fighting in the cage may cause bleeding or even death due to massive internal hemorrhaging. After the tail cutting, wounds must be cauterized to prevent homozygous knockout mice from blood loss and death. This strain is a powerful model for studying coagulopathy, gene therapy methods and function of factor IX mutations. F9-KO mice (Stock No.KO 18046）carry a knockout allele derived from the targeted deletion of exon 8. While F9-KO(2) mice (Stock No.KO 200607) carrying the exon 1-8 deletion. | |||||
| F9-KO(2) | KO | KO, Disease Model | LIVE | KO 200607 | Inquire |
| F9-KO(2) mice (Stock No.KO 200607）carry a knockout allele derived from the targeted deletion of exon 1-8. While F9-KO mice (Stock No.KO 18046) carrying the exon 8 deletion. | |||||
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The F9 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a F9 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What F9 Knockout mouse models are available?
When F9 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is F9 knockout embryonic lethal in mice?
It depends on background and allele design. Some F9 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live F9 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for F9?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.
Related models and routes
Breed this line with ingenious targeting laboratory
Send the F9 Knockout line to a U.S. barrier facility for colony maintenance, cohort production, and complex breeding schemes through mouse breeding services.