Fanca Conditional Knockout mouse models
Fanca, mouse ortholog of human FANCA, encodes the most frequently mutated Fanconi anemia gene product, a scaffolding subunit of the multiprotein FA core complex that, with FANCB, FANCC, FANCE, FANCF, FANCG, FANCL and FAAP proteins, functions as the E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI after the FANCM and FAAP24 sensor detects a stalled replication fork at an interstrand crosslink. Loss abolishes ID2 activation, blocking recruitment of SLX4, XPF and the translesion machinery, producing the diagnostic hypersensitivity to mitomycin C and diepoxybutane and progressive bone marrow failure. Null mice reproduce the cellular crosslinker sensitivity and germ cell attrition but only develop overt marrow failure when combined with aldehyde detoxification alleles such as Aldh2 or Adh5, which is the key design insight for building a faithful hematopoietic model. Because the core complex is nonredundant, single nulls are informative. Use constitutive nulls for crosslink repair biology and compound aldehyde-stress backgrounds for marrow failure endpoints.
Tissue restricted knockout of Fanca keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells.
What Fanca Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fanca conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fanca-Flox | Conditional Knockout | KO/CKO mice | sperm cryopreservation | CKO 205167 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Fanca Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fanca allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fanca Conditional Knockout mouse models are available?
When Fanca Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fanca knockout embryonic lethal in mice?
It depends on background and allele design. Some Fanca germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fanca animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fanca?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.