Fbp2 Knockout mouse models
The muscle-predominant fructose-1,6-bisphosphatase is encoded by Fbp2, ortholog of human FBP2, which hydrolyzes fructose-1,6-bisphosphate during glyconeogenesis, allowing lactate carbon to be resynthesized into glycogen after intense contraction. The Fbp2 protein is markedly more AMP sensitive than the liver isoform and additionally associates with aldolase, mitochondria and nuclei, where it has been reported to stabilize mitochondrial membrane potential and restrain apoptosis, activities separable from gluconeogenic catalysis. The allele fork therefore matters: a conditional null in skeletal muscle or cardiomyocytes tests glycogen resynthesis and recovery kinetics, whereas point mutants that break either the AMP site or the aldolase interaction dissociate catalysis from the reported protective role. Because Fbp1 covers liver and kidney, whole-body glucose homeostasis will often look normal, so avoid systemic glycemia as a primary readout. Instead match allele to muscle glycogen repletion, mitochondrial respirometry and postexercise recovery, using tamoxifen-inducible Cre to bypass developmental adaptation.
A conventional Fbp2 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Fbp2 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fbp2 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fbp2-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 230278 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fbp2 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fbp2 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fbp2 Knockout mouse models are available?
When Fbp2 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fbp2 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fbp2 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fbp2 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fbp2?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.