Fcer1a Knockout mouse models
Fcer1a encodes the alpha chain of the high-affinity IgE receptor, mouse ortholog of human FCER1A, providing the ligand-binding subunit of FcepsilonRI, a tetramer completed by the beta chain MS4A2 and a disulfide-linked FCER1G gamma dimer on mast cells and basophils. Two extracellular Ig-like domains grip the Cepsilon2 to Cepsilon3 region of IgE Fc with picomolar affinity and slow dissociation, so receptors remain chronically occupied; antigen-driven crosslinking of bound IgE aggregates receptors, triggering LYN and FYN phosphorylation of the gamma chain ITAMs, SYK recruitment, LAT and PLCgamma signaling, calcium flux, degranulation of histamine and proteases, eicosanoid generation, and cytokine transcription. Species specificity is the dominant design constraint, since human IgE does not bind mouse FcepsilonRI alpha, which is why humanized FCER1A knockins are required for omalizumab, ligelizumab, and human IgE dependent anaphylaxis studies, whereas conditional deletion with mast cell restricted Cre resolves which effector population drives an allergic endpoint.
A conventional Fcer1a knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Fcer1a Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fcer1a knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fcer1a-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 190798 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fcer1a Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fcer1a allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fcer1a Knockout mouse models are available?
When Fcer1a Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fcer1a knockout embryonic lethal in mice?
It depends on background and allele design. Some Fcer1a germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fcer1a animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fcer1a?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.