Fgf10 Knockout mouse models
Fgf10, ortholog of human FGF10, encodes a mesenchymally expressed paracrine ligand with strong heparan sulfate affinity that signals with high selectivity through the FGFR2b splice variant on adjacent epithelium, driving FRS2 to ERK output and constituting the reciprocal mesenchymal arm of the FGF10 to FGFR2b to SHH loop that governs branching morphogenesis. It is required for lung bud initiation and outgrowth, limb bud induction, and development of salivary, lacrimal, mammary, and pancreatic epithelium, and it maintains adult alveolar and airway progenitors. Germline nulls die at birth with complete lung agenesis and absent limbs, so conditional floxed alleles are mandatory for postnatal regeneration studies. Human heterozygous loss causes lacrimo-auriculo-dento-digital syndrome and aplasia of lacrimal and salivary glands, making hypomorphic or heterozygous designs the closer disease match. The related ligand FGF7 shares FGFR2b and can partially substitute in glandular epithelium, so compound alleles clarify attribution. Pair the flox with Tbx4-rtTA or Dermo1-Cre depending on the tissue endpoint.
Fgf10 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What Fgf10 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fgf10 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fgf10-KO | Knockout | KO/CKO mice | In production — inquire | KO 253250 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fgf10 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgf10 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgf10 Knockout mouse models are available?
When Fgf10 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgf10 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgf10 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgf10 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgf10?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.