Fgf16 Knockout mouse models
Fgf16, ortholog of human FGF16, encodes a secreted FGF9 subfamily ligand that, unlike most paracrine members, dimerizes in a way that limits heparan sulfate binding and diffusion, and it signals preferentially through FGFR1c, FGFR2c, FGFR3c, and FGFR4 with FRS2 to ERK output. Expression concentrates in embryonic and adult cardiomyocytes and brown adipose tissue, where it supports cardiomyocyte proliferation during ventricular growth and modulates hypertrophic and injury responses, and additional signal in developing limb correlates with human phenotypes. Hemizygous human loss-of-function variants cause X-linked metacarpal 4 and 5 fusion, and the mouse locus is likewise X-linked, so hemizygous male, homozygous female, and mosaic heterozygous female comparisons must be built into the design. Nulls are viable, making constitutive deletion tractable for cardiac stress testing, while conditional floxed alleles with Myh6-Cre or Adipoq-Cre attribute effects to muscle versus fat. Substantial overlap with Fgf9 and Fgf20 at shared c-isoform receptors argues for compound alleles when scoring proliferation endpoints.
A conventional Fgf16 knockout removes gene function in all cells that inherit the allele. It is a proven first pass for target validation when redundancy is low.
What Fgf16 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fgf16 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fgf16-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 251414 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Fgf16 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fgf16 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fgf16 Knockout mouse models are available?
When Fgf16 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fgf16 knockout embryonic lethal in mice?
It depends on background and allele design. Some Fgf16 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fgf16 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fgf16?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.